DMSO, methyl sulfoxide, dimethyl sulphoxide
Molecular Formula: C2H6OS
Molecular Weight: 78.13
CAS Number: 67-68-5
Melting Point: 18.45 °C
Boiling Point: 189 °C
Density: 1.1 g/ml
Dielectric Constant: 45
Viscosity: 1.1 centipoises (27 °C)
Sterility: Sterile filtered
Preparation Instructions This product is miscible (disolves completely) in water (1 ml DMSO + 1 ml H2O), yielding a clear, colorless solution.
DMSO is a very hygroscopic liquid. That is to say, it will literally take the water out of the air and should protected from exposure to moisture. If the solution is exposed to water for a prolonged period of time, the DMSO will certainly become contaminated and should not be used. DMSO is also keenly sensitive to UV rays and should be protected and stored in UV resistant glass containers.
DMSO is also soluble in ethanol, acetone, ether, benzene, and chloroform. Your product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.
DMSO super cools quite easily and remelts slowly at room temperature. Which incidentally, is the best way to reconstitute frozen DMSO liquid. DMSO is stable up to 100 °C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.
Scientific information below! Courtesy Sigma Labs
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Nucleases: (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids. Nucleases variously effect single and double stranded breaks in their target molecules. In living organisms, they are essential machinery for many aspects of DNA repair. Defects in certain nucleases can cause genetic instability or immunodeficiency. Nucleases are also extensively used in molecular cloning.
Proteases: (also known as a proteolytic enzyme, peptidase or proteinase) is a type of enzyme that functions mainly to help us digest different kinds of proteins. They break down the bonds by a process known as hydrolysis and convert proteins into smaller chains called peptides or even smaller units called amino acids
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Among its uses in organic synthesis is the oxidation of thiols and disulfides to sulfonic acids.
Other reactions in which DMSO participates include the hydrolysis of epoxides, the thioalkylation of phenols, and the oxidation of primary alcohols, primary halides, and esters of primary alcohols to aldehydes.
Protocols have been reported for the use of DMSO in column-loading buffers for poly(A)+ RNA selection, in buffers for the transformation of competent E. coli, in the polymerase chain reaction (PCR), the amplification of DNA libraries, DNA sequencing, DEAE-dextran mediated transfection of cells, and polybrenemediated DNA transfection.
A procedure that uses DMSO to recover DNA from membrane filters for subsequent PCR amplification has been described. A Capillary electrophoresis technique for DNA Sequencing incorporates 2 M urea with 5% DMSO (v/w), and can be modified to use 100% DMSO as needed.
A study of the contribution of various DMSO Concentrations to melting temperatures in Oligonucleotides has been published. DMSO is also widely utilized in the storage of human and animal cell lines and bacteriophage, as a cryoprotective agent. A protocol to prepare a DMSO solution for freezing cells is as follows:
1) Prepare freezing medium containing culture medium with 10-20% serum and 5-10% DMSO.
2) Remove adherent cells with trypsin or other appropriate means. (For optimal results, cells should be in the log phase of growth.)
3) Gently pellet the cells by centrifugation (10 minutes at 250 × g, 4 °C) and remove the culture medium.
4) Resuspend the cells in the freezing medium at 106 -107 cells/ml.
5) Aliquot into freezing vials.
6) Freeze cells according to standard freezing protocols. Store at -70 °C or below. For cell fusion, a 10% DMSO solution in 40-50% polyethylene glycol (PEG) may be prepared.4